TaqMan microRNA Assay:Article Title: A set of circulating microRNAs belonging to the 14q32 chromosome locus identifies two subgroups of individuals with recent-onset type 1 diabetes.
Article Snippet: .. TAQMAN MICRORNA ASSAY INVITROGEN cat.#4440887 ID 002332 TAQMAN MICRORNA ASSAY INVITROGEN cat.#4440887 ID 000452 TAQMAN MICRORNA ASSAY INVITROGEN cat.#4440887 ID 000572 TAQMAN MICRORNA ASSAY INVITROGEN cat.#4440887 ID 000564 ddPCR EvaGreen Supermix BIORAD 1864034 ddPCR Smx for Probes (no dUTP), 5 3 1mL BIORAD 1863024 DG8 Cartridges and Gaskets BIORAD 1864007 ddPCR Plates 96-Well, Semi-Skirted (25 plates) BIORAD 12001925 PIERCEABLE FOIL HEAT SEAL (no.100) BIORAD 1814040 Droplet Gen Oil for Probes, 10 3 7 mL BIORAD 1863005 Deposited data Small RNA seq First Cohort GEO GSE265980 HTG-Edge seq miRNA First Cohort GEO GSE265976 Small RNA seq Second Cohort GEO GSE265981 Software and algorithms Graphpad Prism v10.0 GraphPad Software (Boston, MA) https://www.graphpad.com/ 2100 Expert Software (vB.02.11) Agilent N/A; RRID:SCR_014466 Illumina Experiment Manager Software (v1.19.1) ILLUMINA N/A; RRID:SCR_021202 BaseSpace v. 1.1.0.64 ILLUMINA N/A; RRID:SCR_011881 GeneGlobe Data Analysis Center QIAGEN N/A ExpressionSuite Software (v1.3) THERMO FISHER N/A; RRID:SCR_021095 R/Bioconductor N/A N/A Other Vacutainer Safety-Lok butterflies BD Biosciences 367282 NovaSeq Xp Flow Cell Dock ILLUMINA 20021663 Vacutainer K2 EDTA BD Biosciences 368861 DNA LoBind Tubes 0.5 mL Eppendorf 0030 108.035 DNA LoBind Tubes 1.5 mL Eppendorf 0030 108.071 DNA LoBind Tubes 2.0 mL Eppendorf 0030 108.058 96 Fast PCR Plate half skirt SARSTEDT 72.1981.202 MicroAmp 96 Optical Adhesive Film 100 cover Thermo Fisher 4311971 Strip 8 flat caps (Xtra-Clear) STARLAB I1400-0900 StarTub Reagent Reservoir (PVC) STARLAB E2310-1000 96 PCR Plate without skirt SARSTEDT 72.1978.202 SimpliAmp Thermal Cycler LIFE TECHNOLOGIES A24811 Qubit Assay Tube Set Thermo Fisher Q32856 Centrifuge MiniSpin Eppendorf 5452000010 Ministar centrifuge VWR 521–2319 NovaSeq 6000 System ILLUMINA 20012850 DynaMag-96 Side Magnet INVITROGEN 12331D DynaMag-2 Magnet INVITROGEN 12321D QUBIT 3.0 spectrofluorometer INVITROGEN N/A 2100 Bioanalyzer Instrument Agilent N/A IKA MS3 S36 Agilent vortex IKA Agilent N/A HTG EdgeSeq Processor HTG N/A HTG EdgeSeq Processor HTG N/A SimpliAmp Thermal Cycler LIFE TECHNOLOGIES N/A (Continued on next page) Cell Reports Medicine 5, 101591, June 18, 2024 e2 ..
RNA Sequencing:Article Title: A set of circulating microRNAs belonging to the 14q32 chromosome locus identifies two subgroups of individuals with recent-onset type 1 diabetes.
Article Snippet: .. TAQMAN MICRORNA ASSAY INVITROGEN cat.#4440887 ID 002332 TAQMAN MICRORNA ASSAY INVITROGEN cat.#4440887 ID 000452 TAQMAN MICRORNA ASSAY INVITROGEN cat.#4440887 ID 000572 TAQMAN MICRORNA ASSAY INVITROGEN cat.#4440887 ID 000564 ddPCR EvaGreen Supermix BIORAD 1864034 ddPCR Smx for Probes (no dUTP), 5 3 1mL BIORAD 1863024 DG8 Cartridges and Gaskets BIORAD 1864007 ddPCR Plates 96-Well, Semi-Skirted (25 plates) BIORAD 12001925 PIERCEABLE FOIL HEAT SEAL (no.100) BIORAD 1814040 Droplet Gen Oil for Probes, 10 3 7 mL BIORAD 1863005 Deposited data Small RNA seq First Cohort GEO GSE265980 HTG-Edge seq miRNA First Cohort GEO GSE265976 Small RNA seq Second Cohort GEO GSE265981 Software and algorithms Graphpad Prism v10.0 GraphPad Software (Boston, MA) https://www.graphpad.com/ 2100 Expert Software (vB.02.11) Agilent N/A; RRID:SCR_014466 Illumina Experiment Manager Software (v1.19.1) ILLUMINA N/A; RRID:SCR_021202 BaseSpace v. 1.1.0.64 ILLUMINA N/A; RRID:SCR_011881 GeneGlobe Data Analysis Center QIAGEN N/A ExpressionSuite Software (v1.3) THERMO FISHER N/A; RRID:SCR_021095 R/Bioconductor N/A N/A Other Vacutainer Safety-Lok butterflies BD Biosciences 367282 NovaSeq Xp Flow Cell Dock ILLUMINA 20021663 Vacutainer K2 EDTA BD Biosciences 368861 DNA LoBind Tubes 0.5 mL Eppendorf 0030 108.035 DNA LoBind Tubes 1.5 mL Eppendorf 0030 108.071 DNA LoBind Tubes 2.0 mL Eppendorf 0030 108.058 96 Fast PCR Plate half skirt SARSTEDT 72.1981.202 MicroAmp 96 Optical Adhesive Film 100 cover Thermo Fisher 4311971 Strip 8 flat caps (Xtra-Clear) STARLAB I1400-0900 StarTub Reagent Reservoir (PVC) STARLAB E2310-1000 96 PCR Plate without skirt SARSTEDT 72.1978.202 SimpliAmp Thermal Cycler LIFE TECHNOLOGIES A24811 Qubit Assay Tube Set Thermo Fisher Q32856 Centrifuge MiniSpin Eppendorf 5452000010 Ministar centrifuge VWR 521–2319 NovaSeq 6000 System ILLUMINA 20012850 DynaMag-96 Side Magnet INVITROGEN 12331D DynaMag-2 Magnet INVITROGEN 12321D QUBIT 3.0 spectrofluorometer INVITROGEN N/A 2100 Bioanalyzer Instrument Agilent N/A IKA MS3 S36 Agilent vortex IKA Agilent N/A HTG EdgeSeq Processor HTG N/A HTG EdgeSeq Processor HTG N/A SimpliAmp Thermal Cycler LIFE TECHNOLOGIES N/A (Continued on next page) Cell Reports Medicine 5, 101591, June 18, 2024 e2 ..
Software:Article Title: A set of circulating microRNAs belonging to the 14q32 chromosome locus identifies two subgroups of individuals with recent-onset type 1 diabetes.
Article Snippet: .. TAQMAN MICRORNA ASSAY INVITROGEN cat.#4440887 ID 002332 TAQMAN MICRORNA ASSAY INVITROGEN cat.#4440887 ID 000452 TAQMAN MICRORNA ASSAY INVITROGEN cat.#4440887 ID 000572 TAQMAN MICRORNA ASSAY INVITROGEN cat.#4440887 ID 000564 ddPCR EvaGreen Supermix BIORAD 1864034 ddPCR Smx for Probes (no dUTP), 5 3 1mL BIORAD 1863024 DG8 Cartridges and Gaskets BIORAD 1864007 ddPCR Plates 96-Well, Semi-Skirted (25 plates) BIORAD 12001925 PIERCEABLE FOIL HEAT SEAL (no.100) BIORAD 1814040 Droplet Gen Oil for Probes, 10 3 7 mL BIORAD 1863005 Deposited data Small RNA seq First Cohort GEO GSE265980 HTG-Edge seq miRNA First Cohort GEO GSE265976 Small RNA seq Second Cohort GEO GSE265981 Software and algorithms Graphpad Prism v10.0 GraphPad Software (Boston, MA) https://www.graphpad.com/ 2100 Expert Software (vB.02.11) Agilent N/A; RRID:SCR_014466 Illumina Experiment Manager Software (v1.19.1) ILLUMINA N/A; RRID:SCR_021202 BaseSpace v. 1.1.0.64 ILLUMINA N/A; RRID:SCR_011881 GeneGlobe Data Analysis Center QIAGEN N/A ExpressionSuite Software (v1.3) THERMO FISHER N/A; RRID:SCR_021095 R/Bioconductor N/A N/A Other Vacutainer Safety-Lok butterflies BD Biosciences 367282 NovaSeq Xp Flow Cell Dock ILLUMINA 20021663 Vacutainer K2 EDTA BD Biosciences 368861 DNA LoBind Tubes 0.5 mL Eppendorf 0030 108.035 DNA LoBind Tubes 1.5 mL Eppendorf 0030 108.071 DNA LoBind Tubes 2.0 mL Eppendorf 0030 108.058 96 Fast PCR Plate half skirt SARSTEDT 72.1981.202 MicroAmp 96 Optical Adhesive Film 100 cover Thermo Fisher 4311971 Strip 8 flat caps (Xtra-Clear) STARLAB I1400-0900 StarTub Reagent Reservoir (PVC) STARLAB E2310-1000 96 PCR Plate without skirt SARSTEDT 72.1978.202 SimpliAmp Thermal Cycler LIFE TECHNOLOGIES A24811 Qubit Assay Tube Set Thermo Fisher Q32856 Centrifuge MiniSpin Eppendorf 5452000010 Ministar centrifuge VWR 521–2319 NovaSeq 6000 System ILLUMINA 20012850 DynaMag-96 Side Magnet INVITROGEN 12331D DynaMag-2 Magnet INVITROGEN 12321D QUBIT 3.0 spectrofluorometer INVITROGEN N/A 2100 Bioanalyzer Instrument Agilent N/A IKA MS3 S36 Agilent vortex IKA Agilent N/A HTG EdgeSeq Processor HTG N/A HTG EdgeSeq Processor HTG N/A SimpliAmp Thermal Cycler LIFE TECHNOLOGIES N/A (Continued on next page) Cell Reports Medicine 5, 101591, June 18, 2024 e2 ..
Polymerase Chain Reaction:Article Title: A set of circulating microRNAs belonging to the 14q32 chromosome locus identifies two subgroups of individuals with recent-onset type 1 diabetes.
Article Snippet: .. TAQMAN MICRORNA ASSAY INVITROGEN cat.#4440887 ID 002332 TAQMAN MICRORNA ASSAY INVITROGEN cat.#4440887 ID 000452 TAQMAN MICRORNA ASSAY INVITROGEN cat.#4440887 ID 000572 TAQMAN MICRORNA ASSAY INVITROGEN cat.#4440887 ID 000564 ddPCR EvaGreen Supermix BIORAD 1864034 ddPCR Smx for Probes (no dUTP), 5 3 1mL BIORAD 1863024 DG8 Cartridges and Gaskets BIORAD 1864007 ddPCR Plates 96-Well, Semi-Skirted (25 plates) BIORAD 12001925 PIERCEABLE FOIL HEAT SEAL (no.100) BIORAD 1814040 Droplet Gen Oil for Probes, 10 3 7 mL BIORAD 1863005 Deposited data Small RNA seq First Cohort GEO GSE265980 HTG-Edge seq miRNA First Cohort GEO GSE265976 Small RNA seq Second Cohort GEO GSE265981 Software and algorithms Graphpad Prism v10.0 GraphPad Software (Boston, MA) https://www.graphpad.com/ 2100 Expert Software (vB.02.11) Agilent N/A; RRID:SCR_014466 Illumina Experiment Manager Software (v1.19.1) ILLUMINA N/A; RRID:SCR_021202 BaseSpace v. 1.1.0.64 ILLUMINA N/A; RRID:SCR_011881 GeneGlobe Data Analysis Center QIAGEN N/A ExpressionSuite Software (v1.3) THERMO FISHER N/A; RRID:SCR_021095 R/Bioconductor N/A N/A Other Vacutainer Safety-Lok butterflies BD Biosciences 367282 NovaSeq Xp Flow Cell Dock ILLUMINA 20021663 Vacutainer K2 EDTA BD Biosciences 368861 DNA LoBind Tubes 0.5 mL Eppendorf 0030 108.035 DNA LoBind Tubes 1.5 mL Eppendorf 0030 108.071 DNA LoBind Tubes 2.0 mL Eppendorf 0030 108.058 96 Fast PCR Plate half skirt SARSTEDT 72.1981.202 MicroAmp 96 Optical Adhesive Film 100 cover Thermo Fisher 4311971 Strip 8 flat caps (Xtra-Clear) STARLAB I1400-0900 StarTub Reagent Reservoir (PVC) STARLAB E2310-1000 96 PCR Plate without skirt SARSTEDT 72.1978.202 SimpliAmp Thermal Cycler LIFE TECHNOLOGIES A24811 Qubit Assay Tube Set Thermo Fisher Q32856 Centrifuge MiniSpin Eppendorf 5452000010 Ministar centrifuge VWR 521–2319 NovaSeq 6000 System ILLUMINA 20012850 DynaMag-96 Side Magnet INVITROGEN 12331D DynaMag-2 Magnet INVITROGEN 12321D QUBIT 3.0 spectrofluorometer INVITROGEN N/A 2100 Bioanalyzer Instrument Agilent N/A IKA MS3 S36 Agilent vortex IKA Agilent N/A HTG EdgeSeq Processor HTG N/A HTG EdgeSeq Processor HTG N/A SimpliAmp Thermal Cycler LIFE TECHNOLOGIES N/A (Continued on next page) Cell Reports Medicine 5, 101591, June 18, 2024 e2 ..
Adhesive:Article Title: A set of circulating microRNAs belonging to the 14q32 chromosome locus identifies two subgroups of individuals with recent-onset type 1 diabetes.
Article Snippet: .. TAQMAN MICRORNA ASSAY INVITROGEN cat.#4440887 ID 002332 TAQMAN MICRORNA ASSAY INVITROGEN cat.#4440887 ID 000452 TAQMAN MICRORNA ASSAY INVITROGEN cat.#4440887 ID 000572 TAQMAN MICRORNA ASSAY INVITROGEN cat.#4440887 ID 000564 ddPCR EvaGreen Supermix BIORAD 1864034 ddPCR Smx for Probes (no dUTP), 5 3 1mL BIORAD 1863024 DG8 Cartridges and Gaskets BIORAD 1864007 ddPCR Plates 96-Well, Semi-Skirted (25 plates) BIORAD 12001925 PIERCEABLE FOIL HEAT SEAL (no.100) BIORAD 1814040 Droplet Gen Oil for Probes, 10 3 7 mL BIORAD 1863005 Deposited data Small RNA seq First Cohort GEO GSE265980 HTG-Edge seq miRNA First Cohort GEO GSE265976 Small RNA seq Second Cohort GEO GSE265981 Software and algorithms Graphpad Prism v10.0 GraphPad Software (Boston, MA) https://www.graphpad.com/ 2100 Expert Software (vB.02.11) Agilent N/A; RRID:SCR_014466 Illumina Experiment Manager Software (v1.19.1) ILLUMINA N/A; RRID:SCR_021202 BaseSpace v. 1.1.0.64 ILLUMINA N/A; RRID:SCR_011881 GeneGlobe Data Analysis Center QIAGEN N/A ExpressionSuite Software (v1.3) THERMO FISHER N/A; RRID:SCR_021095 R/Bioconductor N/A N/A Other Vacutainer Safety-Lok butterflies BD Biosciences 367282 NovaSeq Xp Flow Cell Dock ILLUMINA 20021663 Vacutainer K2 EDTA BD Biosciences 368861 DNA LoBind Tubes 0.5 mL Eppendorf 0030 108.035 DNA LoBind Tubes 1.5 mL Eppendorf 0030 108.071 DNA LoBind Tubes 2.0 mL Eppendorf 0030 108.058 96 Fast PCR Plate half skirt SARSTEDT 72.1981.202 MicroAmp 96 Optical Adhesive Film 100 cover Thermo Fisher 4311971 Strip 8 flat caps (Xtra-Clear) STARLAB I1400-0900 StarTub Reagent Reservoir (PVC) STARLAB E2310-1000 96 PCR Plate without skirt SARSTEDT 72.1978.202 SimpliAmp Thermal Cycler LIFE TECHNOLOGIES A24811 Qubit Assay Tube Set Thermo Fisher Q32856 Centrifuge MiniSpin Eppendorf 5452000010 Ministar centrifuge VWR 521–2319 NovaSeq 6000 System ILLUMINA 20012850 DynaMag-96 Side Magnet INVITROGEN 12331D DynaMag-2 Magnet INVITROGEN 12321D QUBIT 3.0 spectrofluorometer INVITROGEN N/A 2100 Bioanalyzer Instrument Agilent N/A IKA MS3 S36 Agilent vortex IKA Agilent N/A HTG EdgeSeq Processor HTG N/A HTG EdgeSeq Processor HTG N/A SimpliAmp Thermal Cycler LIFE TECHNOLOGIES N/A (Continued on next page) Cell Reports Medicine 5, 101591, June 18, 2024 e2 ..
Stripping Membranes:Article Title: A set of circulating microRNAs belonging to the 14q32 chromosome locus identifies two subgroups of individuals with recent-onset type 1 diabetes.
Article Snippet: .. TAQMAN MICRORNA ASSAY INVITROGEN cat.#4440887 ID 002332 TAQMAN MICRORNA ASSAY INVITROGEN cat.#4440887 ID 000452 TAQMAN MICRORNA ASSAY INVITROGEN cat.#4440887 ID 000572 TAQMAN MICRORNA ASSAY INVITROGEN cat.#4440887 ID 000564 ddPCR EvaGreen Supermix BIORAD 1864034 ddPCR Smx for Probes (no dUTP), 5 3 1mL BIORAD 1863024 DG8 Cartridges and Gaskets BIORAD 1864007 ddPCR Plates 96-Well, Semi-Skirted (25 plates) BIORAD 12001925 PIERCEABLE FOIL HEAT SEAL (no.100) BIORAD 1814040 Droplet Gen Oil for Probes, 10 3 7 mL BIORAD 1863005 Deposited data Small RNA seq First Cohort GEO GSE265980 HTG-Edge seq miRNA First Cohort GEO GSE265976 Small RNA seq Second Cohort GEO GSE265981 Software and algorithms Graphpad Prism v10.0 GraphPad Software (Boston, MA) https://www.graphpad.com/ 2100 Expert Software (vB.02.11) Agilent N/A; RRID:SCR_014466 Illumina Experiment Manager Software (v1.19.1) ILLUMINA N/A; RRID:SCR_021202 BaseSpace v. 1.1.0.64 ILLUMINA N/A; RRID:SCR_011881 GeneGlobe Data Analysis Center QIAGEN N/A ExpressionSuite Software (v1.3) THERMO FISHER N/A; RRID:SCR_021095 R/Bioconductor N/A N/A Other Vacutainer Safety-Lok butterflies BD Biosciences 367282 NovaSeq Xp Flow Cell Dock ILLUMINA 20021663 Vacutainer K2 EDTA BD Biosciences 368861 DNA LoBind Tubes 0.5 mL Eppendorf 0030 108.035 DNA LoBind Tubes 1.5 mL Eppendorf 0030 108.071 DNA LoBind Tubes 2.0 mL Eppendorf 0030 108.058 96 Fast PCR Plate half skirt SARSTEDT 72.1981.202 MicroAmp 96 Optical Adhesive Film 100 cover Thermo Fisher 4311971 Strip 8 flat caps (Xtra-Clear) STARLAB I1400-0900 StarTub Reagent Reservoir (PVC) STARLAB E2310-1000 96 PCR Plate without skirt SARSTEDT 72.1978.202 SimpliAmp Thermal Cycler LIFE TECHNOLOGIES A24811 Qubit Assay Tube Set Thermo Fisher Q32856 Centrifuge MiniSpin Eppendorf 5452000010 Ministar centrifuge VWR 521–2319 NovaSeq 6000 System ILLUMINA 20012850 DynaMag-96 Side Magnet INVITROGEN 12331D DynaMag-2 Magnet INVITROGEN 12321D QUBIT 3.0 spectrofluorometer INVITROGEN N/A 2100 Bioanalyzer Instrument Agilent N/A IKA MS3 S36 Agilent vortex IKA Agilent N/A HTG EdgeSeq Processor HTG N/A HTG EdgeSeq Processor HTG N/A SimpliAmp Thermal Cycler LIFE TECHNOLOGIES N/A (Continued on next page) Cell Reports Medicine 5, 101591, June 18, 2024 e2 ..
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